Alexander Attinger, Antonia Drinnenberg, Can Dong, Charu Ramakrishnan, La’Akea Siverts, Tanya L Daigle, Bosiljka Tasic, Hongkui Zeng, Sean Quirin, Karl Deisseroth, Lisa M Giocomo. Environmental Novelty Modulates Rapid Cortical Plasticity During Navigation. 
bioRxiv 2025.10.21.683723; doi: https://doi.org/10.1101/2025.10.21.683723

Abstract
“In novel environments, animals quickly learn to navigate, and position-correlated spatial representations rapidly emerge in both the retrosplenial cortex (RSC) and primary visual cortex (V1). However, the role of plasticity in building these spatial representations, and how experience modulates this process, are not well understood. Here, we investigated the plasticity of spatial representations with real-time, cellular-resolution read and write control of neural activity using two-photon calcium imaging combined with holographic optogenetic stimulation in mice navigating virtual reality environments. Targeted stimulation of individual layer 2/3 neurons rapidly biased neural activity towards stimulation-paired locations in novel, but not familiar, environments. In contrast, RSC layer 5 neurons exhibited stimulation-induced plasticity regardless of environmental familiarity. These findings reveal a layer-specific, experience-dependent modulation of plasticity and offer a framework for how neocortical spatial representations strike a balance between stability of familiar environments with flexibility for continuous updates of relevant context information.”

Alexander Attinger, Antonia Drinnenberg, Can Dong, Charu Ramakrishnan, La’Akea Siverts, Tanya L Daigle, Bosiljka Tasic, Hongkui Zeng, Sean Quirin, Karl Deisseroth, Lisa M Giocomo. Environmental Novelty Modulates Rapid Cortical Plasticity During Navigation. 
bioRxiv 2025.10.21.683723; doi: https://doi.org/10.1101/2025.10.21.683723